Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection
Guanmin Jiang, Xiaoshuai Renc, Yan Liu, Hongtao Chen, Wei Liu, Zhaowang Guo, Yaqin Zhang, Chaoqun Chen, Jianhui Zhou, Qiang Xiao, Hong Shan
doi: https://doi.org/10.1101/2020.02.25.20027755
Abstract
Background: Coronavirus Disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global threat to public health. Aiming to construct an efficient screening pattern, we comprehensively evaluated the performances of RT-PCR and chest CT in diagnosing COVID-19. Methods: The records including demographics, RT-PCR, and CT from 87 confirmed COVID-19 cases and 481 exclusion cases were collected. The diagnostic accuracy of the pharyngeal swab RT-PCR, CT, combination with the second pharyngeal swab RT-PCR or with CT were evaluated individually. Besides, all the stool RT-PCR results were plotted by time to explore the value of stool RT-PCR.
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