Abstract . Three isocoumarins, including two new compounds, (±) pestalactone D ( 1 ) and pestapyrone F ( 2 ), as well as one known compound, pestapyrone D ( 3 ), were isolated from the culture of the endolichenic Pestalotiopsis rhododendri LF-19-12. The planar structures of all compounds were elucidated by NMR and MS spectra. And the absolute configurations of 1 were confirmed by single crystal X-ray diffraction analysis, indicative of it as a racemate of 4 S /12 S and 4 R...