Cell culture . Request a detailed protocol CHO, CHO K1, and HeLa S3 cells were cultured in minimal essential medium Eagle (α-MEM) and Dulbecco’s modified Eagle’s medium, respectively ( Legrand et al., 2020 ; Lolicato et al., 2022 ; Sparn et al., 2022a ). Both media were supplemented with 10% heat-inactivated fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. α-MEM was further supplemented with 2 mM glutamine. Cell lines were grown at 37°C, in the presence of 5% C...