Introduction . Yeast Cadmium Factor 1 (Ycf1) belongs to the ATP Binding Cassette (ABC) transporter family and sequesters glutathione and glutathione heavy metal conjugates to the vacuole to detoxify reactive oxygen species and heavy metals (Cd 2+ , Se 2+ , Hg 2+ , As 2+ )1 , 2 , 3. In eukaryotes, ABC transporters are divided into five subfamilies (A, B, C, D, and G) with different topologies built around a core architecture made of two nucleotide-binding domains (NBDs) ...